Heterogeneity of Hemin

Heterogeneity of hemin.

Hemin Control of Globin Synthesis

Human mature erythrocyte postribosomal supernatant contains a hemin-reversible translational repressor of globin synthesis as measured in the rabbit reticulocyte cell-free system. Incubation of human supernatant minus hemin at 37” for 2 hours results in conversion of the repressor to a hemin-irreversible form. The presence of 35 pM hemin retards this conversion. Both hemin-reversible and -irrev...

HETEROGENEITY OF MACROPHAGE POPULATIONS: ANTIBODIES DETECTING VARIOUS POPULATIONS OF MACROPHAGES

Two groups of antibodies against mouse resident alveolar and peritoneal macrophages have been raised individually in rabbits by immunization with whole cell suspensions of lavaged alveolar and peritoneal macrophages. The whole antisera reacted positively with neutrophils and lymphocytes. However, upon extensive absorptions with these cells the activity became restricted to macrophages. The...

Hemin control of globin synthesis. Isolation of a hemin-reversible translational repressor from human mature erythrocytes.

Human mature erythrocyte postribosomal supernatant contains a hemin-reversible translational repressor of globin synthesis as measured in the rabbit reticulocyte cell-free system. Incubation of human supernatant minus hemin at 37” for 2 hours results in conversion of the repressor to a hemin-irreversible form. The presence of 35 pM hemin retards this conversion. Both hemin-reversible and -irrev...

Fructation Induces Hemin Degradation in Methemoglobin

Under prolonged diabetic conditions, HbA1c is produced from normal hemoglobin (HbA0) through a non-enzymatic glycosylation or glycation, a process which enhances the hemoglobin susceptibility to be auto-oxidized to methemoglobin (metHb). Here we are reporting that the non-enzymatic reaction between fructose and metHb (metHb fructation) induces significant changes in the gl...